Open AccessPhytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves
Author(s) -
Bárbara Cabral,
Emerson M.S. Siqueira,
Mariana Angélica Oliveira Bitencourt,
Maíra C.J.S. Lima,
Ana Karolina Oliveira Laurentino de Lima,
Caroline Flach Ortmann,
Vítor Clasen Chaves,
Matheus de Freitas Fernandes-Pedrosa,
Hugo Alexandre Oliveira Rocha,
Kátia Castanho Scortecci,
Flávio Henrique Reginatto,
Raquel Brandt Giordani,
Silvana Maria Zucolotto
Publication year - 2016
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2016.02.002
Subject(s) - ellagic acid , chlorogenic acid , antioxidant , chemistry , phytochemical , anti inflammatory , traditional medicine , in vivo , tbars , cytotoxicity , carrageenan , astringent , pharmacology , biochemistry , food science , in vitro , biology , polyphenol , lipid peroxidation , medicine , microbiology and biotechnology , taste
Spondias mombin L., Anacardiaceae, is a plant native of Brazil, where it is known as "cajá". In order to find a potential application for this native species, the anti-inflammatory and antioxidant effects were investigated. The anti-inflammatory activity was evaluated using the in vivo model carrageenan-induced peritonitis in mice. The in vitro antioxidant potential as well the cytotoxicity against 3T3 fibroblast cells also were evaluated. Through High Performance Liquid Chromatography-diode array detector analysis, an analytic method was developed and validated. It allowed the identification and quantification of ellagic acid and chlorogenic acid in hydroethanolic extract of S. mombin leaves. This extract showed anti-inflammatory effect at 100, 200, 300 and 500 mg/kg, however, the ethyl acetate fraction, at 200 mg/kg, showed the highlighted results. Ellagic acid and chlorogenic acid (2.5, 5 and 10 mg/kg) also inhibited the leukocyte migration to the site of inflammation. The extract, fractions and compounds showed significant antioxidant potential when evaluated in different assays. The results shown in this work suggest the anti-inflammatory potential of the leaf extract of S. mombim on peritonitis model induced by carrageenan, it was also observed antioxidant properties associated with an absence of cytotoxicity in cell culture. Further in vivo studies are required to confirm the anti-inflammatories action of S. mombin and its possible anti-inflammatory mechanisms of action