Open AccessEffects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
Author(s) -
Lizandra G. Magalhães,
Thaís C. Lima,
Renato Graciano de Paula,
Enyara Rezende Morais,
Daniela P. Aguiar,
Luiz Gustavo Gardinassi,
Gustavo García,
Rosangela da Silva de Laurentiz,
Vanderlei Rodrigues,
Jairo Kenupp Bastos,
Ademar A. S. Filho,
Ana Patrícia Yatsuda,
Wilson Roberto Cunha,
Márcio Luís Andrade e Silva
Publication year - 2016
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2016.02.001
Subject(s) - schistosoma mansoni , praziquantel , schistosomiasis , schistosoma , biology , in vivo , proteome , schistosoma japonicum , in vitro , immunology , biochemistry , helminths , microbiology and biotechnology
Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (-)-6,6'-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies