Open AccessPhytochemical study and evaluation of cytotoxic and genotoxic properties of extracts from Clusia latipes leaves
Author(s) -
Natalia Bailón-Moscoso,
Juan Carlos Romero-Benavides,
Monserrat Sordo,
Javier Villacís,
Ronald Silva,
Luisa Celi,
Mariano MartínezVázquez,
Patricia OstroskyWegman
Publication year - 2016
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2015.08.014
Subject(s) - friedelin , cytotoxic t cell , chemistry , phytochemical , ethyl acetate , comet assay , hesperidin , in vitro , pharmacology , biochemistry , dna damage , biology , stereochemistry , dna , medicine , alternative medicine , triterpenoid , pathology
Some species of the Clusia genus have been shown to have important biomedical properties, including the ability to inhibit tumor growth in vitro and the usefulness for skin care. In this study, we examined the cytotoxic effect of hexane, ethyl acetate and methanol extracts from Clusia latipes Planch. & Triana, Clusiaceae, leaves on survival of human prostate cancer cells (PC-3), colon cancer cells (RKO), astrocytoma cells (D-384), and breast cancer cells (MCF-7). The ethyl acetate extract displayed the most substantial cytotoxic effect. However, using a Comet assay, we observed that the hexane extract induced a genotoxic effect (DNA damage) on human lymphocytes in an in vitro model. Chromatographic purification of the C. latipes hexane extract led to the isolation and identification of friedelin, friedolan-3-ol, and hesperidin as active cytotoxic compounds in hexane extract, while β-amyrine was identified as an active cytotoxic compound in the ethyl acetate extract of C. latipes, thereby supporting further studies of the molecular mechanisms underlying the effect of these secondary metabolites on cancer cell survival