Open AccessAntimicrobial (including antimollicutes), antioxidant and anticholinesterase activities of Brazilian and Spanish marine organisms – evaluation of extracts and pure compounds
Author(s) -
Éverson Miguel Bianco,
Jéssica Lenita Krug,
Priscila Laiz Zimath,
Aline Kroger,
Camila Jeriane Paganelli,
Ariela Maína Boeder,
Larissa dos Santos,
Adrielli Tenfen,
Suzi Meneses Ribeiro,
Kátia Naomi Kuroshima,
Michele Debiasi Alberton,
Caio Maurício Mendes de Córdova,
Ricardo Andrade Rebelo
Publication year - 2015
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2015.07.018
Subject(s) - antimicrobial , biology , microbiology and biotechnology , traditional medicine , antioxidant , botany , sargassum , algae , biochemistry , medicine
This work describes the antimicrobial, antioxidant and anticholinesterase activities in vitro of organic extracts from fourteen seaweeds, eleven sponges, two ascidians, one bryozoan, and one sea anemone species collected along the Brazilian and Spanish coast, as well as the isolation of the diterpene (4R, 9S, 14S)-4α-acetoxy-9β,14α-dihydroxydolast-1(15),7-diene (1) and halogenated sesquiterpene elatol (2). The most promising antimicrobial results for cell wall bacteria were obtained by extracts from seaweeds Laurencia dendroidea and Sargassum vulgare var. nanun (MIC 250μg/ml), and by the bryozoan Bugula neritina (MIC 62.5μg/ml), both against Staphylococcus aureus. As for antimollicutes, extracts from seaweeds showed results better than the extracts from invertebrates. Almost all seaweeds assayed (92%) exhibited some antimicrobial activity against mollicutes strains (Mycoplasma hominis, Mycoplasma genitalium, Mycoplasma capricolum and Mycoplasma pneumoniae strain FH). From these seaweeds, A1 (Canistrocarpus cervicornis), A11 (Gracilaria sp.) and A4 (Lobophora variegata) showed the best results for M. pneumoniae strain FH (MIC 250μg/ml). Furthermore, compounds 1 and 2 were also assayed against mollicutes strains M. hominis, M. genitalium, M. capricolum, M. pneumoniae strain 129 and M. pneumoniae strain FH, which showed MIC>100μg/ml. Antioxidant activities of extracts from these marine organisms were inactive, except for E7 (from sponge Ircinia sp.), which exhibited moderated antioxidant activities for two methods assayed (IC50 83.0±0.1μg/ml, and 52.0±0.8mg AA/g, respectively). Finally, for the anticholinesterase activity, all the 29 samples evaluated (100%) exhibited some level of activity, with IC50<1000μg/ml. From these, seaweeds extracts were considered more promising than marine invertebrate extracts [A10 (IC50 14.4±0.1μg/ml), A16 (IC50 16.4±0.4μg/ml) and A8 (IC50 14.9±0.5μg/ml)]. The findings of this work are useful for further research aiming at isolation and characterization of active compounds