Open AccessIn vivo potential hypoglycemic and in vitro vasorelaxant effects of Cecropia glaziovii standardized extracts
Author(s) -
Daniela Paula Arend,
Talitha Caldas dos Santos,
Luisa Helena Cazarolli,
Mariana Appel Hort,
Diva Sonaglio,
Ana Lúcia Gomes dos Santos,
Rosa Maria RibeirodoValle,
Fátima Regina Mena Barreto Silva,
Ángela Machado de Campos
Publication year - 2015
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2015.05.010
Subject(s) - chemistry , chlorogenic acid , caffeic acid , alloxan , pharmacology , diabetes mellitus , in vivo , oral administration , biochemistry , food science , medicine , endocrinology , biology , antioxidant , microbiology and biotechnology
The aim of this study is to investigate the effect of Cecropia glaziovii Snethl, Urticaceae, extracts on the oral glucose tolerance curve, on glycemia in alloxan-induced diabetic rats and vasorelaxant effect after the extraction process, and to standardize the extractive solutions. The effects of the process variables and their interactions were calculated in relation to dry residue, pH, total phenolic results and chemical marker content. Furthermore, the effect of the extracts (400mg/kg), chlorogenic (2 or 15mg/kg) and caffeic acids (2mg/kg) were investigated on the oral glucose tolerance curve and on glycemia in alloxan-induced diabetic rats. Oral administration of ethanol extracts 4d20 and 8d20 significantly improved glucose tolerance in the hyperglycemic rats. Chlorogenic and caffeic acids, as well as the association of the compounds were able to significantly reduce glycemia after oral gavage treatments. On the other hand, the aqueous extracts did not alter the glycemia. The aqueous extracts (8020 and 9030) and only the higher dose of chlorogenic acid presented a significant effect on serum glucose lowering in diabetic rats. Additionally, the IC50 reveals that the ethanol extracts presented more potent vasodilator effects than the aqueous extracts in aortic rings. This study shows that C. glaziovii standardized extracts exhibits antihyperglycemic action, is able to improve glucose tolerance and has a potent vascular relaxing effect. These results are probably linked to concentrations of the main phenolic compounds of the extracts