Open AccessAntiproliferative activity, antioxidant capacity and chemical composition of extracts from the leaves and stem of Chresta sphaerocephala
Author(s) -
Larissa Saito da Costa,
Nathalia Luiza Andreazza,
Wallace Ribeiro Corrêa,
Inara Pereira da Cunha,
Ana Lúcia Tasca Góis Ruiz,
João Ernesto de Carvalho,
Elisandra Cristina Schinor,
Diones Aparecida Dias,
Marcos José Salvador
Publication year - 2015
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2015.04.005
Subject(s) - dpph , ethyl acetate , chemistry , hexane , antioxidant , chromatography , methanol , traditional medicine , food science , biochemistry , organic chemistry , medicine
In this study, antiproliferative and antioxidant activities of crude extracts (hexane, ethyl acetate and methanol) from leaves and stem of Chresta sphaerocephala DC., Asteraceae, were investigated. Antiproliferative activity was tested in vitro against ten human cancer cells and against VERO (no cancer cell). Antioxidant activities were determined using DPPH and ORAC-FL assays and the total phenolic content was estimated by Folin–Ciocalteu method. Hexane and ethyl acetate extracts (leaves and stem) exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 50.40 and 250μg/ml. For VERO cell, TGI values were >250μg/ml for all extracts, except to hexane extract of the stem (TGI 80.92μg/ml). In an initial evaluation, ethyl acetate and methanol extracts (leaves and stem) have shown levels of phenolic compounds between 6.94 and 30.96mg GAE/kg in Folin–Ciocalteu assay, DPPH free-radical scavenging activity with SC50 in the range of 75.22 and 400μg/ml and antioxidant capacity between 290.08 and 1088μmol TE/g of extract in ORAC-FL assay. HPLC-DAD and ESI-MS analysis allowed the identification of flavonoids in the methanol extract from the leaves of C. sphaerocephala. Three steroids and nine triterpenoids were identified in the bioactive hexane extracts using HRGC