Open AccessAcute genotoxicity analysis in vivo of the aqueous extract of Maytenus guyanensis Amazonian chichuá
Author(s) -
Dionatas Ulises de Oliveira Meneguetti,
Renato Abreu Lima,
Francisco Carlos da Silva,
Guilherme Matos Passarini,
João Bezerra Facundo,
Rubiani de Cássia Pagotto,
Júlio Sancho Linhares Teixeira Militão,
Valdir Alves Facundo
Publication year - 2015
Publication title -
revista brasileira de farmacognosia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 46
eISSN - 1981-528X
pISSN - 0102-695X
DOI - 10.1016/j.bjp.2015.03.003
Subject(s) - genotoxicity , traditional medicine , celastraceae , pharmacology , comet assay , in vivo , micronucleus test , biology , toxicology , chemistry , medicine , toxicity , dna damage , botany , microbiology and biotechnology , biochemistry , dna
The species Maytenus guyanensis Klotzsch ex Reissek, Celastraceae, present a wide variety of possible pharmacological activities and its roots and stems are used by popular medicine in the western Amazon rainforest. Few studies have demonstrated the genotoxic safety of the popular use of this species, and owing to this, the present study aimed to perform an analysis of the acute genotoxicity in vivo of the aqueous extract of M. guyanensis. Male and female mice from Mus musculus species, of weights ranging from 20 to 40g, organized in eight groups with different treatments were used. The aqueous extracts of the bark of M. guyanensis were administered orally by gavage with 0.1ml of the test substance per 10g of the animal, followed by performance of comet assay in peripheral blood, PCE/NCE correlation and occurrence of micronuclei in the bone marrow. It was found that the aqueous extract of M. guyanensis, with ten times higher concentration than those used in ethnopharmacology, did not present genotoxic effect and, moreover, it has antigenotoxic action in mice treated acutely. Further studies regarding bioaccumulation and chronic effects of this species are suggested, in order to improve the understanding of its mechanism of action, ensuring the efficacy and safety of its utilization and developing phytotherapics and drugs