Open AccessThe prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in Inner Mongolia, China
Author(s) -
Haiying Niu,
Hao Yu,
Tangping Hu,
Gailin Tian,
Lixia Zhang,
Xiang Guo,
Hai Hu,
Zhanli Wang
Publication year - 2016
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.643
H-Index - 64
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1016/j.bjm.2016.04.003
Subject(s) - aminoglycoside , microbiology and biotechnology , virulence , biology , enterococcus , gentamicin , enterococcus faecium , enterococcus faecalis , streptomycin , antibiotic resistance , gene , antibiotics , genetics , escherichia coli
This study highlights the prevalence of aminoglycoside-modifying enzyme genes and virulence determinants among clinical enterococci with high-level aminoglycoside resistance in Inner Mongolia, China. Screening for high-level aminoglycoside resistance against 117 enterococcal clinical isolates was performed using the agar-screening method. Out of the 117 enterococcal isolates, 46 were selected for further detection and determination of the distribution of aminoglycoside-modifying enzyme-encoding genes and virulence determinants using polymerase chain reaction -based methods. Enterococcus faecium and Enterococcus faecalis were identified as the species of greatest clinical importance. The aac(6')-Ie-aph(2″)-Ia and ant(6')-Ia genes were found to be the most common aminoglycoside-modifying enzyme genes among high-level gentamicin resistance and high-level streptomycin resistance isolates, respectively. Moreover, gelE was the most common virulence gene among high-level aminoglycoside resistance isolates. Compared to Enterococcus faecium, Enterococcus faecalis harbored multiple virulence determinants. The results further indicated no correlation between aminoglycoside-modifying enzyme gene profiles and the distribution of virulence genes among the enterococcal isolates with high-level gentamicin resistance or high-level streptomycin resistance evaluated in our study.