Open AccessIsolation of chemical constituents from Nonomuraea species: In vitro and in silico evaluation of its antibacterial properties
Author(s) -
Pachaiyappan Saravana Kumar,
Antony Stalin,
R. Lakshmi Sundaram,
Veeramuthu Duraipandiyan,
Naïf Abdullah Al-Dhabi,
P. Yuvaraj,
K. Balakrishna,
S. Ignacimuthu
Publication year - 2017
Publication title -
beni-seuf university journal of basic and applied sciences /beni-suef university journal of basic and applied sciences
Language(s) - English
Resource type - Journals
eISSN - 2314-8543
pISSN - 2314-8535
DOI - 10.1016/j.bjbas.2016.12.004
Subject(s) - antibacterial activity , phthalic acid , chemistry , in silico , chromatography , strain (injury) , docking (animal) , 16s ribosomal rna , chemical structure , stereochemistry , biochemistry , bacteria , organic chemistry , biology , gene , medicine , genetics , nursing , anatomy
An actinomycetes strain VAS16 was isolated from the soil collected from the agricultural field of Vengodu. The strain was identified as Nonomuraea species based on morphological, physiological and biochemical characteristics along with 16S rRNA gene sequence analysis. The active extract was purified over silica gel column chromatography. The structure of the active compound was elucidated using FT-IR, EI-MS, 1H NMR and 13C NMR spectra; it was identified as phthalic acid. Phthalic acid obtained from the strain Nonomuraea sp. VAS16 showed significant antibacterial activity against Yersinia enterocolitica and Vibrio parahaemolyticus, with a MIC value of 31.25 μg/mL. In silico docking analysis was carried out using GlcN-6-P synthesis as a target. It showed that the compound had minimum binding energy of −6.55 KJ/mol and had good affinity. This compound could be probed further for its usefulness as a drug molecule