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The protein kinase kin1 is required for cellular symmetry in fission yeast
Author(s) -
Carbona Stéphanie,
Allix Caroline,
Philippe Michel,
Goff Xavier
Publication year - 2004
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/j.biolcel.2003.12.004
Subject(s) - cytokinesis , schizosaccharomyces pombe , biology , microbiology and biotechnology , cell cortex , interphase , cell division , mitosis , schizosaccharomyces , cell cycle , cell polarity , actin , cytoskeleton , spindle pole body , microtubule , actin remodeling , actin cytoskeleton , saccharomyces cerevisiae , cell , spindle apparatus , yeast , genetics
The fission yeast Schizosaccharomyces pombe is a highly polarized unicellular eukaryote with two opposite growing poles in which F‐actin cytoskeleton is focused. The KIN1/PAR‐1/MARK protein family is composed of conserved eukaryotic serine/threonine kinases which are involved in cell polarity, microtubule stability or cell cycle regulation. Here, we investigate the function of the fission yeast KIN1/PAR‐1/MARK member, kin1p. Using a deletion allele ( kin1 Δ), we show that kin1 mutation promotes a delay in septation. Kin1p regulates the structure of the new cell end after cytokinesis by modulating cell wall remodeling. Abnormal shaped interphase kin1 Δ cells misplace F‐actin patches and the premitotic nucleus. Thus, mitotic kin1 Δ cells misposition the F‐actin ring assembly site that is dependent on the position of the interphase nucleus. The resulting asymmetric cell division produces daughter cells with distinct shapes. Overexpressed kin1p accumulates asymmetrically at the cell cortex and affects cell shape, F‐actin organization and microtubules. Our results suggest that correct dosage of kin1p at the cortex is required for spatial organization of the fission yeast cell.