Open AccessResveratrol pretreatment reduces circulating inflammatory interleukins in CCl 4 -induced hepatotoxicity rats
Author(s) -
Yasser I. Kandil,
Anwar Maraqa,
Ghaleb A. Oriquat,
Ziad Shraideh
Publication year - 2017
Publication title -
bulletin of faculty of pharmacy, cairo university /bulletin of faculty of pharmacy, cairo university
Language(s) - English
Resource type - Journals
eISSN - 2090-9101
pISSN - 1110-0931
DOI - 10.1016/j.bfopcu.2017.09.005
Subject(s) - ccl4 , oxidative stress , resveratrol , glutathione , medicine , bilirubin , inflammation , interleukin , pharmacology , immunology , cytokine , chemistry , carbon tetrachloride , enzyme , biochemistry , organic chemistry
Liver diseases represent one of major health burdens worldwide, both oxidative stress and inflammation play crucial roles in the development of liver diseases. Resveratrol (RSV), a naturally occurring compound, has recently been shown to exert anti-oxidative and anti-inflammatory actions. This work aimed to investigate the prophylactic effect of RSV against CCl4-induced hepatotoxicity in experimental rats in terms of inflammatory interleukins and histopathological changes. Twenty-four adult male rats were divided into three equal groups: group A: Negative control, received oral vehicle, group B: Positive control, received oral vehicle for 6â¯days and on 6th day injected with single dose of CCl4, and group C: RSV-treated group, received oral RSV (25â¯mg/kg/day) for 6â¯days and on 6th day injected with single dose of CCl4. 24â¯h after induction of hepatotoxicity by CCl4, all rats were sacrificed; liver was excised for histopathological studies and blood samples were collected. ALT, AST, ALP, total bilirubin, IL-1β, IL-6, and IL-10 were determined in plasma while total GSH, MDA, GPx, and SOD were assayed in liver homogenate. Plasma levels of ALT, AST, ALP, total-bilirubin, IL-1β, and IL-6 were significantly increased 24â¯h after induction of hepatotoxicity by CCl4 while IL-10 decreased. Pretreatment of rats with RSV prevented these changes. The histopathological changes were less obvious in livers of RSV-treated rats in comparative to positive control. In conclusion, RSV has a prophylactic effect against hepatotoxicity induced by CCl4 through decreasing the inflammatory interleukins level. Keywords: Resveratrol, Hepatotoxicity, Interleukins, Inflammatio