Open AccessTLC-densitometric and first derivative spectrophotometric methods for determination of cefoxitin-sodium in presence of its alkali-induced degradation product
Author(s) -
Khalid A.M. Attia,
Omar Y. Abdelaziz,
Nancy Magdy,
Gamal A. Mohamed
Publication year - 2017
Publication title -
bulletin of faculty of pharmacy, cairo university /bulletin of faculty of pharmacy, cairo university
Language(s) - English
Resource type - Journals
eISSN - 2090-9101
pISSN - 1110-0931
DOI - 10.1016/j.bfopcu.2017.09.004
Subject(s) - alkali metal , derivative (finance) , cefoxitin , degradation (telecommunications) , chromatography , chemistry , sodium , analytical chemistry (journal) , organic chemistry , computer science , biology , genetics , telecommunications , bacteria , financial economics , economics , staphylococcus aureus
Two simple and accurate methods were developed for quantitative estimation of cefoxitin sodium in presence of its open ring alkali-induced degradation product. The first method was based on TLC separation of the drug from its alkali-induced degradation product followed by densitometric measurement of the intact drug at 254 nm. The proposed method obeys Beerâs law in range (1â20 µg/band). The second method was first derivative (1D) spectrophotometric method; was based on measurement of amplitude of first order spectra of cefoxitin sodium (in the range of 4â36 µg mLâ1) at 282 nm which showed zero crossing point of its degradation product. The two methods were validated according to ICH guideline for accuracy, precision and were successfully applied for the determination of the drug in pure form and pharmaceutical preparation in presence of its alkali-induced degradation product. Keywords: Cefoxitin sodium, Stability indicating methods, Thin layer chromatography (TLC)-densitometry, First derivative (1D) spectrophotometric metho