Open AccessUtility of picric acid and 2,4 dinitrophenol as chromogenic reagents for visible spectrophotometric quantification of alogliptin
Author(s) -
Ashwini Kumar,
Tukaram Reddy,
Chandra Bala Sekharan
Publication year - 2017
Publication title -
bulletin of faculty of pharmacy, cairo university /bulletin of faculty of pharmacy, cairo university
Language(s) - English
Resource type - Journals
eISSN - 2090-9101
pISSN - 1110-0931
DOI - 10.1016/j.bfopcu.2017.02.002
Subject(s) - picric acid , alogliptin , chromogenic , chemistry , chromatography , chloroform , calibration curve , titration , reagent , detection limit , organic chemistry , medicine , dipeptidyl peptidase 4 , type 2 diabetes , diabetes mellitus , endocrinology
Two simple and sensitive visible spectrophotometric methods (A and B) were developed for the determination of alogliptin in bulk and in its tablet dosage forms. The methods use the reaction of alogliptin with picric acid (method A) or 2,4 dinitrophenol (method B) in the chloroform medium. The complex of alogliptin with picric acid (method A) or 2,4 dinitrophenol (method B) showed λmax at 415 nm and 430 nm respectively. The different conditions affecting the formation and stability of the complexes were optimized. The methods were validated statistically according to ICH. The calibration curve is linear over the concentration range of 10–60 μg ml−1 and 10–50 μg ml−1 for methods A and B, respectively. The proposed methods were successfully applied for the assay of alogliptin in tablets with good recoveries. Interference was not observed from common tablet excipients