Open AccessMicellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma
Author(s) -
Mohamed Rizk,
Safaa S. Toubar,
Maha M. Abou ElAlamin,
Marwa M. Azab
Publication year - 2014
Publication title -
bulletin of faculty of pharmacy, cairo university /bulletin of faculty of pharmacy, cairo university
Language(s) - English
Resource type - Journals
eISSN - 2090-9101
pISSN - 1110-0931
DOI - 10.1016/j.bfopcu.2014.03.002
Subject(s) - triethylamine , chromatography , chemistry , repeatability , phosphoric acid , high performance liquid chromatography , sodium dodecyl sulfate , sodium , organic chemistry
A micellar liquid chromatographic method was developed for the determination of sertaconazole and terconazole in bulk, dosage forms and human plasma using intersil cyano column and mobile phase consisting of 0.1M sodium dodecyl sulphate, 20% 1-propanol, and 0.3% triethylamine in 0.02M ortho-phosphoric acid (pH 4) at 225nm. Different chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc. The method was validated over the concentration ranges 8–40 and 16–80μg/ml, for sertaconazole and terconazole, respectively. The method was sensitive with limits of detection of 1.24 and 1.67μg/ml for sertaconazole and terconazole in bulk, respectively. Inter and intra-day results showed % RSD<0.9% and 1.55% for sertaconazole and terconazole, respectively. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as a stability-indicating method for the determination of drugs under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters