Open AccessValidated spectrophotometric methods for the evaluation of Oseltamivir counterfeit pharmaceutical capsules
Author(s) -
Rasha M. Youssef,
Fawzy A. ElYazbi,
E. Khamis,
Sameh E. Younis
Publication year - 2014
Publication title -
bulletin of faculty of pharmacy, cairo university /bulletin of faculty of pharmacy, cairo university
Language(s) - English
Resource type - Journals
eISSN - 2090-9101
pISSN - 1110-0931
DOI - 10.1016/j.bfopcu.2013.12.001
Subject(s) - absorbance , spectrophotometry , repeatability , chromatography , significant difference , accuracy and precision , chemistry , dosage form , ascorbic acid , analytical chemistry (journal) , materials science , mathematics , statistics , food science
Four rapid, reliable and economical spectrophotometric methods have been established for the quantitative determination of Oseltamivir phosphate (OST) without the interference of ascorbic acid (ASC) found in some of its counterfeit capsules. The first method involves the use of derivative spectrophotometry with the zero-crossing technique where OST was easily determined using its 1D (Δλ=3) at 219nm. The second method is based on a first-order derivative ratio spectrophotometry (1DD, Δλ=5) where 218nm was selected for its quantification, while the third method applies a more advanced spectrophotometric method based on the ratio difference spectrophotometry (RD) in which the difference in absorbance ratio was measured between 217 and 210nm. In the fourth method, difference spectrophotometric method (ΔA) is applied by subtracting absorbance at 252 from that at 263nm where the difference in absorbance was zero for ASC. The proposed methods were validated for linearity, accuracy, precision and selectivity. Synthetic mixtures of different proportions and commercial capsules were assayed by the proposed methods and the results revealed good accuracy and repeatability of the developed methods