Open AccessScaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor
Author(s) -
Hewaida F. El-Sedawy,
Mohamed Hussein,
Tamer Essam,
O. M. El–Tayeb,
Fatma H.A. Mohammad
Publication year - 2013
Publication title -
bulletin of faculty of pharmacy, cairo university /bulletin of faculty of pharmacy, cairo university
Language(s) - English
Resource type - Journals
eISSN - 2090-9101
pISSN - 1110-0931
DOI - 10.1016/j.bfopcu.2013.02.002
Subject(s) - aeration , fermentation , yield (engineering) , industrial fermentation , chemistry , saturation (graph theory) , pulp and paper industry , food science , materials science , mathematics , organic chemistry , engineering , metallurgy , combinatorics
Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. The use of aeration level 1.5 vvm increased the yield by 16.6% (from 13.81 to 16.1 g/l) upon controlling the temperature at 28 °C. Adjustment of the aeration level at 1.5 vvm for 3 days then controlling the dissolved oxygen (DO) at 30% saturation further increased the yield to 17.8 g/l. The optimum pH was 6.5 for 3 days then 7 thereafter when a production yield of 16.1 g/l was recorded using an aeration rate of 1.5 vvm. Controlling the pH at constant value (6.5 or 7) all through the fermentation process decreased the yield by 5–21%. Controlling the temperature at 30 °C for 3 days then 28 °C thereafter slightly increased the yield by 5% upon using an aeration rate of 1 vvm while it decreased upon using an aeration rate of 1.5 vvm. Integration of the most optimum conditions increased the production yield by 22% from 13.81 to 17.8 g/l