Open AccessAntibacterial activity of naringenin-rich fraction of pigeon pea leaves toward Salmonella thypi
Author(s) -
Sarifa Agus,
Suminar Setiati Achmadi,
Nisa Rachmania Mubarik
Publication year - 2017
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2017.07.019
Subject(s) - naringenin , ethyl acetate , chemistry , salmonella , antibacterial activity , fraction (chemistry) , chloramphenicol , cajanus , extraction (chemistry) , minimum inhibitory concentration , salmonella typhi , chromatography , flavonoid , biology , bacteria , antibiotics , biochemistry , escherichia coli , antioxidant , horticulture , gene , genetics
Objective: To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi).Methods: The leaf sample was powdered and macerated with methanol and fractioned by liquid–liquid extraction using ethyl acetate. The fraction was chromatographed and the isolates were identified for major component with liquid chromatography-mass spectrometry and the antibacterial activity was tested against S. thypi by Kirby–Bauer method.Results: Subfraction 1 from the ethyl acetate fraction formed a yellowish solid with m/z 272, identified as naringenin. The naringenin-rich fraction shows fairly well inhibitory toward S. thypi in comparison with chloramphenicol.Conclusions: Naringenin shows antibacterial activity and can be developed to treat typhoid