Open AccessRourea cuspidata : Chemical composition and hypoglycemic activity
Author(s) -
Manuela M. Laikowski,
Paulo Roberto dos Santos,
Débora Menezes de Souza,
Luciane Minetto,
Natalia Girondi,
Camila C. Pires,
Gisiele Alano,
Mariana RoeschEly,
Leandro Tasso,
Sidnei Moura
Publication year - 2017
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2017.07.015
Subject(s) - glibenclamide , streptozotocin , diabetes mellitus , chemistry , insulin , medicine , pharmacology , traditional medicine , endocrinology
Objective: To investigate the antidiabetic effect of Rourea cuspidata hydroalcoholic stem extract in normal and streptozotocin-induced diabetic rats.Methods: In order to evaluate the chemical composition, different extracts from stem in ascending solvent order of polarity were prepared. The extracts were analyzed by high resolution mass spectrometry and 7 compounds were identified, including hyperin, an important and already reported active compound in the literature. Hyperin was also quantified by HPLC-UV in all the extracts. The hydroalcoholic stem extract (Ss5), which showed the highest concentration of hyperin, was administered to STZ-induced diabetes rats to evaluate the potential hypoglycemic activity. Total cholesterol, HDL, triglycerides, ALT and AST were also evaluated. In the present study, the effects of oral administration of hydroalcoholic stem extract (200 mg/kg b. wt.) for 28 days on the level of serum glucose, total cholesterol, HDL, triglycerides, aspartate amino transferase (AST) and alanine amino transferase (ALT) in normal and streptozotocin-induced diabetic rats were evaluated. Histopathological changes in diabetic rats' pancreas were also studied.Results: The extract exposition demonstrated hypoglycemic effect like the drug control glibenclamide. The extract was able to increase the HDL levels. Histopathological study on diabetic rats' pancreas after extract treatment showed morphological alterations in STZ-induced diabetes rats, which were apparently restored after extract treatment.Conclusions: This work demonstrates the potential use of R. cuspidata as hypoglycemic agent in the treatment of diabetes