Open AccessAntioxidant antileishmanial cytotoxic and antimicrobial activities of a local plant Myrtus nivellei from Algeria Sahara
Author(s) -
Farah Ramdane,
Rym Essid,
Nadia Farès,
Dahmane El Ouassis,
Sana Aziz,
Mahfoud Hadj Mahammed,
Mohamed Didi Ould El Hadj,
Férid Limam
Publication year - 2017
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2017.07.011
Subject(s) - phytochemical , antimicrobial , rutin , traditional medicine , gallic acid , dpph , chemistry , antioxidant , ethyl acetate , agar diffusion test , phytochemistry , polyphenol , chromatography , organic chemistry , biochemistry , medicine , escherichia coli , gene
Objective: To study the phytochemical constituents and in vitro biological activities of hydromethanolic extract and fractions from Algerian Sahara Myrtus nivellei (M. nivellei) collected in Hoggar region and to identify the active fraction that can act as an alternative of commonly used antibiotics and as antileishmanial or antioxidant agents.Methods: Phytochemical screening of M. nivellei aerial parts was realised according to the literature. Extract was firstly prepared by using aqueous methanol then fractionated with ethyl acetate and butanol solvents. Total phenolics, tannis and flavonoids, of the hydromethanolic extract and their fractions were determined by Folin–Ciocalteu method as gallic acid equivalents and by aluminium chloride as rutin equivalent respectively. Extract and fractions were tested for their antimicrobial and antiparasital activities against standard bacteria using agar diffusion method and two kinds of leishmania visceral and cutaneous. The antioxidant activities were realized using phosphomolybdenum, FRAP and DPPH tests.Results: Preliminary phytochemical screening exhibited the presence of flavonoids, tannins, saponins, and alkaloids. The experimental results showed that plant extract and fractions were high in phenolic compounds and exhibited an important role as antioxidant, antimicrobial and had a moderate antileishmanial activity.Conclusions: These observations lead us toward more studies in this field, so that we can get more benefits from our local Algerian medicinal plants