Open AccessIsolation and identification of compounds from Phaleria macrocarpa (Scheff.) Boerl fruit extract
Author(s) -
Emanuel Dani Ramdani,
Ujiatmi Dwi Marlupi,
James Sinambela,
Raymond R. Tjandrawinata
Publication year - 2017
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2016.12.018
Subject(s) - heteronuclear molecule , ethyl acetate , chemistry , hexane , proton nmr , two dimensional nuclear magnetic resonance spectroscopy , chromatography , traditional medicine , organic chemistry , stereochemistry , nuclear magnetic resonance spectroscopy , medicine
Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa (P. macrocarpa) fruit ethanolic extract.Methods: Dried fruit of P. macrocarpa was extracted with 90% ethanol and partitioned between n-hexane/H2O and ethyl acetate/H2O. The organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance (NMR), carbon-13 NMR)], 2D-NMR (heteronuclear multiple-quantum correlation and heteronuclear multiple-bond correlation), and mass spectrum.Results: Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P. macrocarpa fruit resulted in isolation of nine compounds.Conclusions: A new compound was isolated and identified as glyceryl pentacosanoate. Also, two xanthones, which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are firstly reported to be isolated from P. macrocarpa