Open AccessIn vitro and in vivo susceptibility of Leishmania major to some medicinal plants
Author(s) -
Fatemeh Maleki,
Mitra Zarebavani,
Mehdi Mohebali,
Mohammad Saaid Dayer,
Fateme Hajialiani,
Fatemeh Tabatabaie
Publication year - 2017
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2016.11.008
Subject(s) - in vivo , medicine , in vitro , leishmaniasis , traditional medicine , leishmania , pharmacology , cutaneous leishmaniasis , leishmania donovani , parasite hosting , leishmania major , biology , immunology , visceral leishmaniasis , microbiology and biotechnology , world wide web , computer science , biochemistry
ObjectiveTo evaluate the efficacy of some medicinal plants and systemic glucantime in a comparative manner against the causative agent of cutaneous leishmaniasis both in vitro and in BALB/c mice.MethodsFor in vivo testing, inbred mice were challenged with Leishmania major parasites and the resultant ulcers were treated with extract based-ointments applied topically two times per day for a period of 20 days. A group of 56 mice were randomly divided into 7 subgroups. The control group received the ointment void of extracts, whereas the reference group received glucantime only. The efficacy of treatments was evaluated by measuring ulcer diameter, parasite burden and NO production.ResultsOur results indicated that plant extract based-ointments were effective in reducing ulcer size and parasite burden in spleens, but their effects did not differ significantly from that of glucantime. The plant extracts tested in this study were able to increase NO production that helped parasite suppression.ConclusionsOur findings indicate that the tested plant extracts are effective against Leishmania major both during in vitro and in vivo experiments, but further researches are required to recommend a potential plant extract as an alternative drug