Open AccessGC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco) (Moraceae) leaves
Author(s) -
Franelyne P. Casuga,
Agnes L. Castillo,
Mary Jho Anne T. Corpuz
Publication year - 2016
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2016.08.015
Subject(s) - moraceae , broussonetia , chemistry , gas chromatography–mass spectrometry , mass spectrometry , traditional medicine , ethyl acetate , biological activity , hexane , gas chromatography , chemical constituents , chromatography , botany , biology , biochemistry , medicine , in vitro
ObjectiveTo investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica (Blanco) (Moraceae) (B. luzonica), an endemic plant in the Philippines.MethodsThe air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane, ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry.ResultsQualitative determination of the different biologically active compounds from crude extracts of B. luzonica using gas chromatography–mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the three different extracts possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the leaves of B. luzonica.ConclusionsThe three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies