Open AccessCytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract
Author(s) -
Muhammad Zubair,
Syariful Anam,
Subehan Lallo
Publication year - 2016
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2016.04.014
Subject(s) - ethyl acetate , phytochemical , alkaloid , hela , chemistry , chromatography , acetic anhydride , terpenoid , thin layer chromatography , mtt assay , traditional medicine , biochemistry , in vitro , stereochemistry , medicine , catalysis
ObjectiveTo evaluate the cytotoxic activity of wood extracts of Lunasia amara Blanco (L. amara) and to perform further phytochemical standardization.MethodsThe wood extracts of L. amara were assessed for cytotoxic activity by in vitro tetrazolium bromide (MTT) method against two human cancer cell lines, cervical cancer cells (HeLa) and breast cancer cells (T47D). Thin layer chromatography, Dragendorf, acetic anhydride-sulfuric acid and ferric chloride were used to detect alkaloids, steroids and polyphenols, respectively. Furthermore, quantitative determination of total alkaloid by ultra fast liquid chromatography-photodiode array detection using lunacrine as a marker compound was performed as well.ResultsThe ethyl acetate extract exhibited higher cell-growth inhibition than methanol and n-hexane extracts on HeLa and T47D cancer line cells with the IC50 of 71.15 and 79.04 μg/mL, respectively. Total alkaloid in ethyl acetate extract was counted as (10.46 ± 0.28)% (w/w), while lunacrine determined by ultra fast liquid chromatography-photodiode array detection method was found to be (3.55 ± 0.26)% (w/w).ConclusionsThe high total alkaloid and lunacrine concentration on the extract confirm the potential cytotoxic property of ethyl acetate wood extract of L. amara