Open AccessInfluence of CD133+ expression on patients' survival and resistance of CD133+ cells to anti-tumor reagents in gastric cancer
Author(s) -
Dehu Chen,
Ruiqi Lu,
Xiaochun Ni,
Jiemin Wu,
Shoulian Wang,
Bo-jian Jiang,
Jiwei Yu
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2015.09.005
Subject(s) - cancer cell , cancer stem cell , cancer research , apoptosis , cell culture , cancer , cell , in vitro , immunomagnetic separation , chemistry , biology , stem cell , immunology , microbiology and biotechnology , biochemistry , genetics
ObjectiveTo investigate the influence of CD133+ expression on patients' survival and resistance of CD133+ cells to anti-tumor agents in gastric cancer (GC).MethodsInfluence of CD133 expression on prognosis was analyzed employing samples from patients with GC. GC cell lines were utilized to separate CD133+ and CD133− subpopulations by immunomagnetic separation and to analyze the biological features of two subpopulations in vitro and in vivo, especially in resistant to anti-tumor reagents and its apoptotic mechanism.ResultsThe lower CD133+ group showed a significantly better survival compared with the higher CD133+ group. The highest content of CD133+ subpopulations for KATO-III cells had stronger proliferative ability than CD133− subpopulations. A single CD133+ cell was capable of generating new cell colony and the tumorigenicity rate in nude mice was 100% for CD133+ clonal spheres or for CD133+ cells, but 0% for CD133− cells. Furthermore, the higher expression levels of Oct-4, Sox-2, Musashi-1 and ABCG2 in CD133+ clonal spheres were identified compared with CD133+ cells or CD133− cells. Under the treatment of anti-tumor reagents, CD133+ cells had lower suppression rates compared with CD133− cells while lower level of Bcl-2 and higher level of Bax were found in CD133+ cells compared with CD133− cells.ConclusionsThe patients with lower CD133+ expression had a better survival. Enriched CD133+ cells in clonal sphere shared the ability to be self-renewable, proliferative, tumorigenic and resistant to anti-tumor agents as probably regulated by Bcl-2 and Bax