Open AccessCocktail of Theileria equi antigens for detecting infection in equines
Author(s) -
Shimaa Abd El‒Salam El‒Sayed,
Mohamed Abdo Rizk,
Mohamad Alaa Terkawi,
Ahmed Abdelmoniem Mousa,
El Said El Shirbini El Said,
Ghada M. El-Sayed,
Mohamed A. Fouda,
Naoki Yokoyama,
Ikuo Igarashi
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2015.09.001
Subject(s) - virology , antigen , theileria , biology , medicine , veterinary medicine , immunology , world wide web , computer science , parasite hosting
ObjectiveTo use two diagnostic antigens belonging to the frequently associated in Theileria domain, Theileria equi (T. equi) protein 82 (Te 82) and T. equi 104 kDa microneme-rhoptry antigen precursor (Te 43), to diagnose T. equi infection in horses as compared with equi merozoite antigen-2 (EMA-2).MethodsIn the current study, we applied a cocktail-ELISA containing two antigens (EMA-2 + Te 82) to diagnose T. equi infection either in experimentally infected horses or in field infection.ResultsOur findings have revealed that a cocktail formula of EMA-2 + Te 82 provided a more practical and sensitive diagnostic candidate for diagnosing T. equi infection in horses as compared with Te 82 or Te 43 alone.ConclusionsThe ELISA technique using a cocktail formula of EMA-2 + Te 82 offers a practical and sensitive diagnostic tool for diagnosing T. equi infection in horses and using of this promising cocktail formula will be applicable for epidemiological surveys and will help control the infection in horses