Open AccessTinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines
Author(s) -
Hataipan Phienwej,
Ih-si Swasdichira,
Surattana Amnuoypol,
Prasit Pavasant,
Piyamas Sumrejkanchanakij
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2015.07.001
Subject(s) - cell culture , matrix metalloproteinase , head and neck squamous cell carcinoma , cell migration , wound healing , cell , cytotoxicity , cancer research , chemistry , microbiology and biotechnology , medicine , biology , pharmacology , immunology , in vitro , cancer , head and neck cancer , biochemistry , genetics
ObjectiveTo investigate the effect of Tinospora crispa (T. crispa) extract on matrix metalloproteinase-13 (MMP-13) expression and cell migration.MethodsThe cytotoxicity of T. crispa extract was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on head and neck squamous cell carcinoma (HNSCC) cell lines. The effect on expression of MMP-13 was analysed by RT-PCR and ELISA. The migration was assessed by wound healing assay.ResultsMMP-13 mRNA was highly expressed in the metastatic human HNSCC cell lines, HN22 and HSC-3. T. crispa extract at a concentration of 100.0 μg/mL caused about 50% reduction of cell survival. T. crispa extract at a non-toxic concentration of 12.5, 25.0 and 50.0 μg/mL significantly suppressed MMP-13 mRNA expression and secreted MMP-13 in both HN22 and HSC-3. The expression of tissue inhibitors of metalloproteinase-2 (TIMP-2) by HSC-3 cells was attenuated by 25.0 and 50.0 μg/mL of T. crispa extract. Addition of the extract to cells in a wound healing assay showed inhibition of cell migration by HN22 cells.ConclusionsThese data suggest that T. crispa could be considered as a potential therapeutic drug to prevent metastasis of HNSCC