Open AccessA method for microbial decontamination of Acanthamoeba cultures using the peritoneal cavity of mice
Author(s) -
Daniella de Sousa Mendes Moreira Alves,
Rodrigo Gurgel-Gonçalves,
Patrícia Albuquerque,
César Augusto Cuba-Cuba,
Maria Imaculada MunizJunqueira,
Selma Aparecida Souza Kückelhaus
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2015.06.002
Subject(s) - acanthamoeba , microbiology and biotechnology , peritoneal cavity , inoculation , human decontamination , bacteria , strain (injury) , biology , microorganism , medicine , pathology , immunology , anatomy , genetics
Objective: To evaluate whether the inoculation of contaminated cultures in the peritoneal cavity of mice could implement decontamination of Acanthamoeba cultures.Methods: Suspensions of Acanthamoeba, Acanthamoeba polyphaga ATCC 30461, or Acanthamoeba spp. isolated from soil (UnB13 strain) were inoculated in the peritoneal cavity of Swiss mice (n = 24). After 1, 6, 12, or 24 h of exposure the peritoneal cavity was washed and assessed for the presence of bacteria, fungi, and Acanthamoeba.Results: After 1 h of intraperitoneal inoculation at least 97% of the bacteria and 96% of the fungi (P < 0.05) and 99% of the bacteria (P < 0.05) were successfully eliminated from the ATCC 30461 strain and from the soil isolate UnB13 strain, respectively. This method also allowed the recovery of most trophozoites and cysts from both Acanthamoeba cultures at the end of 24 h.Conclusions: Our data demonstrated that this technique has great potential for decontamination of Acanthamoeba cultures in a short period of time