Open AccessOccurrence and characterization of toxigenic Bacillus cereus in food and infant feces
Author(s) -
Sameer R. Organji,
Hussein H. Abulreesh,
Кhaled Elbanna,
Gamal Osman,
Manal Khider
Publication year - 2015
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.1016/j.apjtb.2015.04.004
Subject(s) - bacillus cereus , cereus , raw milk , food science , microbiology and biotechnology , biology , enterotoxin , penicillin , feces , bacteria , antibiotics , escherichia coli , biochemistry , genetics , gene
ObjectiveTo investigate the true incidence of Bacillus cereus (B. cereus) in food and children diarrhea cases.MethodsA total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the presence of B. cereus by selective plating on mannitol-egg-yolk-polymyxin agar. Confirmation of B. cereus was carried out by biochemical tests and PCR. Identification of non-B. cereus isolates was carried out by 16S rDNA sequencing. Antimicrobial susceptibility was done by disk diffusion method.ResultsOverall 35 samples (31.8%, n = 110) yielded Bacillus-like growth. Of which 19 samples (54.28%) were positive for B. cereus. All isolates were positive for enterotoxin production. No psychrotolerant B. cereus strains were detected in all samples. All B. cereus isolates were resistant to penicillin G, but susceptible to vancomycin, erythromycin and clindamycin.ConclusionsThe results of this study confirm the importance of including B. cereus in disease control and prevention programs, as well as in routine clinical and food quality control laboratories in both Saudi Arabia and Egypt