Open AccessIn vitro polyembryony induction in a critically endangered fern, Pteris tripartita Sw
Author(s) -
Baskaran Xavier Ravi
Publication year - 2016
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/j.apjr.2016.06.012
Subject(s) - sporophyte , gametophyte , polyembryony , biology , botany , spore , fern , microbiology and biotechnology , embryo , pollen
Objective: An efficient in vitro protocol was established during the development of polyembryony in the spore derived gametophyte of Pteris tripartita Sw.Methods: Sterilized spores were germinated in half strength Murashige and Skoog (MS) basal media to produce gametophyte at in vitro condition. Three-month-old gametophytes were sub-cultured in half strength MS medium with 6-benzylaminopurine (BAP). All inoculated cultures were incubated and noticed every month for polyembryony.Results: Multiple numbers (Di, Tri, Tetra, Hexa, and Octa) of the juvenile sporophyte per spore derived gametophyte were observed at 3 mg/L of BAP in half strength MS basal media, which augmented with 30% sucrose. Hexa polyembryony or juvenile sporophytes was noticed on both 3 and 4 mg/L of BAP, which developed 1.00 and 0.76 cm lengths, respectively. At 4 mg/L of BAP in MS culture medium, various numbers (Di, Tri, Tetra and Hexa) of juvenile sporophytes per gametophyte were formed and showed 1.03, 0.66, 0.53 and 0.76 cm of sporophyte lengths, correspondingly.Conclusion: An in vitro developed polyembryony or juvenile sporophyte exhibited normal growth in their morphological structure