Open AccessEvaluation of polymorphonuclear (PMN) cells in cervical sample as a diagnostic technique for detection of subclinical endometritis in dairy cattle
Author(s) -
Mohammad Rahim Ahmadi,
Ali Kadivar,
M. Vatankhah
Publication year - 2016
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/j.apjr.2016.06.011
Subject(s) - endometritis , subclinical infection , medicine , dairy cattle , receiver operating characteristic , gynecology , biology , zoology , pregnancy , genetics
Objective: This study evaluated the cervical sampling as an easy and safe method for the diagnosis of subclinical endometritis in dairy cattle.Methods: One hundred ninety seven lactating Holstein cows were examined at 26–32 d in milk (DIM) for diagnosis of endometritis. Differential cellular counts were also made from stained smears of the cervical mucosa. Using the Receiver/Response Operating Characteristics (ROC) curve, presence of >17.5% polymorphonuclear (PMN) cells was calculated for detection of subclinical endometritis with sensitivity and specificity of 56.5% and 83.3% respectively.Results: Cows with subclinical endometritis had significantly more open days and all service conception rate than normal cows. The results of survival analysis showed that normal cows became pregnant at a significantly faster rate than cows with subclinical endometritis.Conclusions: The results of the present study introduced the cervical smear sampling as an easy and suitable method for diagnosis of subclinical endometritis in dairy cattle