Open AccessNatural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters
Author(s) -
Reda I. El-Sheshtawy,
Diya A. El-Badry,
Gamal A. El-Sisy,
Walid S. El-Nattat,
Amal M. Abo Almaaty
Publication year - 2016
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/j.apjr.2016.06.004
Subject(s) - cryoprotectant , extender , sperm , acrosome , cryopreservation , andrology , semen , membrane integrity , biology , sperm motility , honey bee , zoology , chemistry , botany , biochemistry , medicine , membrane , fishery , embryo , organic chemistry , polyurethane
Objective: To investigate the effect of extender supplementation with different concentrations of a honey bee on post-thawed sperm motility, viability index, membrane and acrosome integrities in Arab stallion.Methods: Five ejaculates from each of four Arabian stallions were subjected to cryopreservation with a modified INRA-82, without any supplementation (control) or supplemented with 1%, 2%, 3%, 4% and 5% honey bee. After thawing, all samples were maintained at 37 °C, while analyses were performed at 0, 1, and 2 and 3 h. Sperm motility percentage, viability index percentage, membrane integrity percentage and acrosome integrity percentage of each sample were determined by conventional laboratory methods.Results: Relative to the control group, supplementation with honey (2%, 3% and 4% significantly improved (P < 0.01 at least) post-thaw sperm motility, viability index (P < 0.001 at least) and had a positive effect on membrane integrity and intact acrosome percentage (P < 0.001 at least) at 0, 1, 2 and 3 h post-thawing. For all semen parameters, the lower concentration of honey (1%) and higher concentration (5%) did not show significant differences (P > 0.05) compared with the control.Conclusion: Honey bee supplementation in Arab stallion semen extenders provided a better protection of sperm parameters against cryopreservation injury, in comparison to the control groups