Open AccessReproductive toxicity of aqueous wood-ash extract of Azadirachta indica (neem) on male albino mice
Author(s) -
Timothy Auta,
A. T. Hassan
Publication year - 2016
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/j.apjr.2016.01.005
Subject(s) - gonadosomatic index , azadirachta , sperm , sperm motility , andrology , testosterone (patch) , biology , toxicity , histopathology , reproductive toxicity , spermatogenesis , reproduction , motility , endocrinology , medicine , botany , pathology , fecundity , population , ecology , genetics , environmental health
Objective: To evaluate the reproductive toxicity of aqueous wood ash extract of Azadirachta indica (A. indica) in male albino mice.Methods: Four different dose levels of 0, 5, 50 and 100 mg/kg body weight were administered to 20 male mice, with five mice per group for seven days that were sacrificed 35 days thereafter. Gonadosomatic index, sperm motility, sperm count, sperm morphology, serum follicle stimulating hormone (FSH), leuitinizing hormone (LH) and testosterone assay, and histopathology of testes were carried out.Results: Though no toxic effect on testicular weight, FSH, LH and testosterone (P > 0.05), significant decrease in sperm motility, live/dead sperm and sperm count, with significant increase of abnormal sperm were recorded (P < 0.05). Dose dependent histopathological damage of testes was obtained (P < 0.05).Conclusion: Aqueous wood ash extract of A. indica have damaging effects on sperms and testicular tissues, which could impair reproduction