Open AccessDiet-induced obesity alters kinematics of rat spermatozoa
Author(s) -
Ibukun P. Oyeyipo,
Pieter Johann Maartens,
Stefan S. du Plessis
Publication year - 2015
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/j.apjr.2015.06.008
Subject(s) - sperm , motility , andrology , sperm motility , endocrinology , staining , medicine , biology , chemistry , microbiology and biotechnology , genetics
Objective: To investigate the effect of DIO on the kinematics and viability of spermatozoa in an albino rat model.Methods: Sperm suspensions from normal (Control) and diet-induced obese (DIO) Wistar rats were collected and incubated for various times (30, 60, 120 or 180 min at 37 °C). Motility parameters were analyzed with computer-aided sperm analysis (CASA), while viability was assessed by means of a dye exclusion staining technique (eosin/nigrosin).Results: Results reveal that there was a significant time dependent decrease (P < 0.05) in progressive motility, curvilinear velocity and beat cross-frequency after 60 min, while amplitude of lateral head displacement and sperm viability was significantly reduced (P < 0.05) after 120 min in the DIO group compared to control spermatozoa.Conclusions: These results provided evidence that obesity is detrimental to sperm parameter in rats possibly through increased testicular temperature as a result of a rise in fat deposition