Open AccessIn vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol
Author(s) -
Ansoumane Kourouma,
Peng Duan,
Hady Keita,
Aidogie Osamuyimen,
Suqin Qi,
Chao Quan,
Tingting Yu,
Kecheng Yang
Publication year - 2015
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/j.apjr.2015.05.002
Subject(s) - sperm , andrology , sperm motility , epididymis , intraperitoneal injection , nonylphenol , motility , malondialdehyde , male infertility , spermatogenesis , oxidative stress , medicine , endocrinology , chemistry , biology , infertility , microbiology and biotechnology , environmental chemistry , pregnancy , genetics
Objective: To explore the mechanism by which nonylphenol (NP) interferes with male infertility through evaluation of its effects on epididymal sperm of adult male rats.Methods: Twenty four Sprague–Dawley (SD) rats were used as epididymal sperm donors. Previously rats were administrated with NP (0, 2, 10 and 50 mg/kg) body weight respectively in corn oil every forty-eight hours by intraperitoneal injection for 30 days. Computer assisted sperm analysis (CASA) was used to determine parameters of sperm. The sperm morphology examination was conducted with a high resolution microscope.Results: Results indicated that exposure to NP has no effect on body weight, while testes weights were significantly decreased. Computer assisted sperm analysis (CASA) showed significant decline in the percentage of motile spermatozoa (P < 0.001), STR and LIN (P < 0.01), significant increase in ALH (P < 0.001), while significant decline in BCF (P < 0.001) respectively. Plasma LDH was significantly increased while; plasma γ-GT activity was significantly decreased. H2O2 production and malondialdehyde (MDA) were significantly increased. The Plasma CAT, GSH-Px and SOD activities were significantly decreased.Conclusions: This concludes that NP leads oxidative stress in the epididymal sperm of rats. Moreover, NP can disrupt sperm motility and alterations in the sperm morphology