Open AccessAntioxidant and anticancer activities of Plectranthus stocksii Hook. f. leaf and stem extracts
Author(s) -
Kasipandi Muniyandi,
E. Olusȩgun George,
Vekataramana Mudili,
Naveen Kumar Kalagatur,
Allen Joseph Anthuvan,
K. S. Murali Krishna,
Parimelazhagan Thangaraj,
Natarajan Gopalan
Publication year - 2017
Publication title -
agriculture and natural resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.319
H-Index - 13
eISSN - 2468-1458
pISSN - 2452-316X
DOI - 10.1016/j.anres.2016.07.007
Subject(s) - gallic acid , chemistry , trolox , antioxidant , rutin , ethyl acetate , dpph , traditional medicine , food science , caffeic acid , chromatography , biochemistry , medicine
The properties of Plectranthus stocksii—a well-known folk medicinal plant—were investigated. The plant extracts were successively extracted and tested for phytochemicals using high performance liquid chromatography, while antioxidant and anticancerous properties were assessed using MCF-7, Caco-2 and RAW 264.7 cancerous cell line models. The methanolic extract of leaves showed higher concentrations of total phenolics (415.41 mg gallic acid equivalents/g extract) and tannins (177.53 mg gallic acid equivalents/g extract) contents than other studied extracts. In the case of flavonoids, ethyl acetate extract of leaf (LEA) showed a higher concentration (777.11 mg rutin equivalents/g extract) and was also found to have better antioxidant activity against stable radical 2,2-diphenylpicrylhydrazyl (3.46 μg/mL), 2,2′azinobis (3-ethylbenzothiozoline-6-sulfonic acid) disodium salt radical (27.41 mM Trolox equivalent/g extract) and superoxide (24.16 μg/mL) radicals and showed better IC50 (the concentration of the sample at which the inhibition rate reaches 50%) values on MCF-7 (48.874 μg/mL) and Caco-2 (36.088 μg/mL) cancerous cell line models. The immense anti-oxidant potential of P. stocksii leaf and stem extracts could be utilized as a good source of natural, anti-oxidant supplement in food to defend against oxidative-stress-related disorders and more generally in the food safety industry