Open AccessChemical composition of essential oil and hexane extract and antioxidant activity of various extracts of Acmella uliginosa (Sw.) Cass flowers from Indonesia
Author(s) -
Askal Maimulyanti,
Anton Restu Prihadi
Publication year - 2016
Publication title -
agriculture and natural resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.319
H-Index - 13
eISSN - 2468-1458
pISSN - 2452-316X
DOI - 10.1016/j.anres.2015.11.002
Subject(s) - dpph , chemistry , antioxidant , traditional medicine , ethyl acetate , essential oil , food science , hexane , botany , chromatography , organic chemistry , biology , medicine
Medicinal plants are rich sources of natural antioxidant which are used in the prevention and treatment of disease like artherosclerosis, heart stroke, diabetes and cancer and to delay the process of aging. Acmella uliginosa (Sw.) Cass is an edible herb traditionally used in the treatment of many diseases. Analysis of volatile components in the flower extract used gas chromatography-mass spectrometry. The results showed the main components of the essential oil were caryophyllene (21.27%), caryophyllene oxide (15.49%), and 3-carene (10.73%). The main components of the hexane extract were N-isobutyl-2E,6Z,8E-decatrienamide (37.80%), α-pinene (4.98%) and hexadacanoic acid-methyl ester (4.78%). The antioxidant activity of A. uliginosa (Sw.) Cass flower from Indonesia was determined using 1,1, diphenyl-2-picryl hydrazine (DPPH) free radical scavenging assay. The IC50 (defined as the total antioxidant necessary to decrease the initial DPPH radical by 50%) of extracts was calculated. A comparative study determined that A. uliginosa (Sw.) Cass in methanol extract showed higher antioxidant potential (IC50 = 96.83 μg/mL) compared to ethyl acetate extract (IC50 = 123.46 μg/mL) and n-hexane extract (905.92 μg/mL) against DPPH free radicals