
Imbalanced dietary methionine-to-sulfur amino acid ratio can affect amino acid profiles, antioxidant capacity, and intestinal morphology of piglets
Author(s) -
Miaomiao Bai,
Lei Wang,
Hongnan Liu,
Kang Xu,
Jinping Deng,
Ruilin Huang,
Yulong Yin
Publication year - 2020
Publication title -
animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 28
eISSN - 2405-6545
pISSN - 2405-6383
DOI - 10.1016/j.aninu.2020.03.009
Subject(s) - methionine , ileum , jejunum , duodenum , amino acid , medicine , fish meal , antioxidant , biology , endocrinology , soybean meal , glutathione peroxidase , glutathione , meal , zoology , biochemistry , food science , superoxide dismutase , enzyme , fish <actinopterygii> , ecology , raw material , fishery
Animal protein sources such as fishmeal and plasma powder are excellent and indispensable sources of energy, amino acids, and minerals in animal production. Amino acid imbalance, especially methionine-to-sulfur amino acid (Met:SAA) ratio, caused by an imbalance of animal protein meal leads to growth restriction. This study was conducted to evaluate the effects of imbalanced Met:SAA ratio supplementation of different animal protein source diets on growth performance, plasma amino acid profiles, antioxidant capacity and intestinal morphology in a piglet model. Twenty-four weaned piglets (castrated males; BW = 10.46 ± 0.34 kg), assigned randomly into 3 groups (8 piglets/group), were fed for 28 d. Three experimental diets of equal energy and crude protein levels were as follows: 1) a corn-soybean basal diet with a Met:SAA ratio at 0.51 (BD); 2) a plasma powder diet with a low Met:SAA ratio at 0.41 (L-MR); 3) a fishmeal diet with a high Met:SAA ratio at 0.61 (H-MR). Results revealed that compared to BD, L-MR significantly decreased ( P < 0.05) the activities of plasma total antioxidant capacity and glutathione peroxidase, plasma amino acid profiles, and significantly reduced ( P < 0.05) villus height and crypt depth in the duodenum and jejunum. Additionally, L-MR significantly reduced ( P < 0.05) the mRNA expression level of solute carrier family 7 member 9 ( SlC7A9 ) in the ileum, and significantly increased ( P < 0.05) mRNA expression levels of zonula occludens-1 ( ZO-1 ) in the duodenum, and Claudin-1 , ZO-1, sodium-coupled neutral amino acid transporters 2 ( SNAT2 ) and SlC7A7 in the jejunum. H-MR significantly increased ( P < 0.05) plasma SAA levels, and significantly reduced ( P < 0.05) average daily feed intake, villus height, and villus height-to-crypt depth (VH:CD) ratio in the ileum compared to BD. In conclusion, L-MR may result in oxidative stress and villous atrophy but proves beneficial in improving intestinal barrier function and the activity of amino acid transporters for compensatory growth. H-MR may impair intestinal growth and development for weaned piglets. The research provides a guidance on the adequate Met:SAA ratio (0.51) supplementation in diet structure for weaned piglets.