Open AccessA Saccharomyces cerevisiae RC016-based feed additive reduces liver toxicity, residual aflatoxin B1 levels and positively influences intestinal morphology in broiler chickens fed chronic aflatoxin B1-contaminated diets
Author(s) -
Valeria Poloni,
Alejandra Paola Magnoli,
Analía Fochesato,
Andrea Cristofolini,
Matías Daniel Caverzán,
Cecilia Inés Merkis,
Mariana Montenegro,
Lilia Renée Cavaglieri
Publication year - 2020
Publication title -
animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 28
eISSN - 2405-6545
pISSN - 2405-6383
DOI - 10.1016/j.aninu.2019.11.006
Subject(s) - broiler , aflatoxin , biology , histopathology , hyperplasia , crypt , zoology , animal feed , small intestine , toxicity , medicine , endocrinology , food science , pathology
The present study was conducted to investigate the ability of Saccharomyces cerevisiae RC016 (Sc)-based feed additive to reduce liver toxicity, residual aflatoxin B 1 (AFB 1 ) levels and influence intestinal structure in broiler chickens fed chronic aflatoxin B 1 -contaminated diets. A total of 100 one-day-old male commercial line (Ross) broiler chickens were divided into 4 treatments, with 5 pens per treatment and 5 broiler chickens per pen. Birds were randomly assigned to 4 treatments, which were namely treatment 1 (T 1 ), control diet (CD); T 2 , CD + Sc at 1 g/kg; T 3 , CD + AFB 1 at 100 μg/kg; T 4 , CD + Sc at 1 g/kg + AFB 1 at 100 μg/kg. The liver histopathology of broiler chickens fed diets with AFB 1 showed diffused microvacuolar fatty degeneration. The addition of Sc showed normal hepatocytes similar to the control. The small intestine villi from AFB 1 group showed atrophy, hyperplasia of goblet cells, prominent inflammatory infiltrate and oedema. In contrast, the small intestine villi from birds that received the yeast plus AFB 1 showed an absence of inflammatory infiltrate, and atrophy; moreover, a lower number of goblet cells compared to the groups with AFB 1 was observed. The morphometric intestine studies showed that a significant decrease ( P < 0.05) in the crypt depth values when Sc was applied to AFB 1 -contaminated diets. Although the intestinal villus height and apparent adsorption area did not show significant differences ( P > 0.05), there was a tendency to improve these parameters. The residual levels of AFB 1 in livers were significantly reduced ( P < 0.05) in the presence of the yeast. The present work demonstrated that the addition of Sc alone or in combination with AFB 1 in the broiler chicken diets had a beneficial effect in counteracting the toxic effects of AFB 1 in livers besides improving the histomorphometric parameters and modulating the toxic effect of AFB 1 in the intestine.