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205 upregulation of nedl after ngf withdrawal from pc12 cells
Author(s) -
Hamnér S.,
Olsson PA.,
Bäckström S.,
Lindholm D.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80394-7
Subject(s) - citation , downregulation and upregulation , library science , computer science , psychology , information retrieval , biology , genetics , gene
We are interested in the specification of neuronal phenotypes during forebrain development. Using retroviral vectors in vitro, we have shown that neuronal precursor cells of the mammalian neocortex have the potential to generate both glutamatergic projection neurons and GABAergic interneurons (G&z et al., 1995). Composition of the progeny is influenced during the final cell cycle (Gatz and Bolz, 1994) by cytokines and transmitters For example, the transmitter GABA inhibits the generation of GABAergic neurons. Thus, the type of neuron generated is instructed by the local environment of the precursor cells. We then used a short-term aggregation assay to evaluate whether precusor cells from different brain regions mix or segregate in different compartments. The results revealed prominent segregation of cells from different forebrain regions of both mammals and birds. This segregation is developmentally rcylated and mediated by Ca-dependent adhesion molecules The region-specific segregation is correlated to the patterned expression of transcription factors (Pax6/Emx/Otx/Dlx). In Pax6null mutations (Sey-mice) the segregation is attenuated and overexpression of Otxl affects adhesion of cortical but not striatal cells. These results suggest that region-specilic adhesion could serve as the cellular link between the genetic patterning and the compartmentalization of brain regions.