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193 Changes in the activity of vibrissal column induced by neonetal depletion of serotonin in rat
Author(s) -
Djavadian R.,
Turlejski K.,
Kossut M.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80382-0
Subject(s) - column (typography) , citation , library science , serotonin , psychology , history , computer science , medicine , telecommunications , receptor , frame (networking)
Dopaminergic projections from the midbrain play an important role for the development of the basal ganglia. The present study is concerned with the question whether dopamine (DA) affects morphological development of striatal GABAergic neurons and which signal recognitton and transduction mechanisms are involved in the control of differentiation. Striatal cells from embryonic day 17 rat fetuses were grown for 7 days and treated with DA or selective Dl-or D2-like receptor agonists (SKF 38393 or quinpirole, respectively) for 1 h. At various times after termination of treatment, cells were immunostained for growth associated protein 43 (E-50) or Fos-like proteins. Morphological parameters including growth cones, neurite length, numbers of bifurcations, and soma size per neuron were assessed. DA as well as SKF 38393, but not quinpirole, increased the numbers of growth cones, total process length and arborization. This effect took several hours to evolve, remained stable for at least 24 hours, and could be blocked by the Dl-like receptor antagonist SCH 23390 or by cycloheximide. Activation of D l-like but not DZ-like receptors also increased the number of Fos-positive neurons. This effect was restrictet to B-50 negative neurons. Our data demonstrate for the first time a positive regulatory effect of DA on striatal morphogenesis and establish the existence of a signaling pathway encompassing D&like receptors but not necessarily induction of c-fos. Supported by the Deursche Forschungsgenleinschafr.