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135 Neuron‐formation by neuroectodermal progenitor cells immortalized by p53‐deficiency
Author(s) -
Schlett K.,
Herberth B.,
Varju P.,
Madarasz E.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80325-x
Subject(s) - citation , progenitor cell , library science , progenitor , computer science , information retrieval , biology , stem cell , genetics
IMMORTALIZED BY PS3-DEFICIENCY CLONAL PROGENITORS GENERATED FROM ADULT RAT HIPPOCAMPUS MIGRATE AND DIFFERENTIATE AFTER GRAFTING INTO EMBRYONIC BRAIN J.O. Suhonen, J. Takahashi, M. Takahashi. T. Palmer, J. Ray and F.H. Gage. Laboratory of GenetIcs, The Salk Institute, La Jolla, CA 92037-1099 U.S.A. Adult hippocampal progenitors can be cultured for over two year in the presence of basic fibroblast growth factor (FGF-2). When grafted back to adult rat brain, they can migrate to specific structures and undergo site-specific neuronal differentiation. Now we have isolated clones of normal diploid multipotent progenitors from adult rat hippocampus and genetically marked them with a retrovirus vector to express E. Coli L.ucZ gene in the nucleus of the cells. To examine the developmental capacity of one of these clonal adult progenitor cell lines in viva the cells were implanted into embryonic (E17) rat brain. At 9 to 40 days postnatal age. brains were fixed and processed for O-Gal immunoreactivity and neuronal and glial markers. The progenitors implanted into the neuroepithelium of telencephalon and mesencephalon migrated into the developing hippocampal formation and cerebellar cortex where they acquired region-specific morphologies and expressed site-specific neuronal markers, i.e. calbindin and NeuN. Grafted progenitors were found in various other areas undergoing neurogenesis at or after the time of implantation, i.e. striatum, septum, and late developing nuclei of midbram. These findings indicate that clonal adult hippocampal progemtors grafted into the fetal brain migrate and integrate into the host brain cytoarchitecture and they can undergo site-specific neurogenesis during embryonic rat brain development. Supported by grants from NIA, NINDS, the Medical Research Council of the Academy of Finland and Japan Eye Bank.