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122 Tnf receptors (p55 and p75) are expressed in the normal adult mouse brain
Author(s) -
Tchélingérian J.L.,
Le Saux F.,
Jacque C.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80312-1
Subject(s) - citation , receptor , library science , classics , medicine , art , computer science
As previously shown central neurons are able to synthesize both the TNFa mRNA (Neuroreport 5, 1994) and protein (Neuron 10, 1993) after a hippocampal injury in the adult mouse brain. As the expression of the TNF-Rs in the normal as well as in injured brain tissue is not known. it was important to identify the cells which would be susceptible to express these molecules in vivo. Indeed, TNFa binds to two types of receptors, the ~55 (type I) and the ~75 (type II) TNF receptors which are bighly homologous in their extracellular domains. In this study we have used a nonradioactive in situ hybridization method to detect the expression of mRNAs encoding for the TNF receptors in normal and injured adult mouse brain. Probes specific for each receptor were labeled with digoxigenin. In the normal brain mRNAs encoding for each receptor were expressed in neurons in the hippocampus and in different brain areas. A non-neuronal expression of both receptors was observed in meninges, in white matter tracts and in ventral sub-pial glia. We hypothesize that the TNFa which is produced by neuronal cells after brain injury can interact in autocrinc and paracrine fashions witb its specific receptors shown to be also expressed in central neurons.