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115 Gaba transporters during the development of the embryonic chick telencephalon
Author(s) -
Lundgren P.,
Mattsson M.O.,
Johansson L.,
Brorsson A.,
Sellström A.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80305-4
Subject(s) - cerebrum , citation , embryonic stem cell , library science , computer science , biology , neuroscience , genetics , gene , central nervous system
We have previously reported that the survival of mouse cerebellar granule cells is independent of chronic depolarization. In this regard they differ from their rat counterparts. In the present study we measured mRNA concentration for the GluR2 subunit and the relative concentrations of the GluRl-4 mRNA in parallel cultures of mouse and rat cerebellar granule cells. The cultures were grown under depolarizing and nondepolarizing conditions. The concentration of GluR2 mRNA was measured by competitive RT-PCR and was normalized to the concentration of mRNA for the constitutively expressed histone gene H3.3, measured within the same culture. The relative concentrations of mRNA for GluRl4 were measured by restriction enzyme analysis of a PCR product containing cDNA corresponding to all four subtypes. At 4 days, the concentrations of GluR2 and total GluR mRNA were higher in mouse than in rat cultures, independent of cell depolarization. However, whereas at 7 days the concentrations were unchanged in mouse and non-depolarized rat cultures, the concentrations increased in depolarized rat cultures. In conclusion, we suggest that a fairly high AMPA receptor mRNA concentration is correlated to cerebellar granule cell survival in culture. (Supported by a grant from the Danish Medical Research Council).

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