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107 The trophic, co‐trophic, and instructive roles of TGF‐βs for central and peripheral neurons
Author(s) -
Unsicker K.,
Farkas L.,
Huber K.,
Jordan J.,
SuterCrazzolara C.,
Hötten G.,
Pohl J.,
Krieglstein K.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80297-8
Subject(s) - trophic level , citation , library science , information retrieval , combinatorics , computer science , mathematics , biology , ecology
Neumnal growth cones respond to changes in internal calcium and it has been suggested that localized calcium changes can guide axons. We previously showed that calcineurin is involved in growth cone motility and may transduce localized caIcium changes to direct movement (Chang ef al.. 1995). Microscale chromophore assisted laser inactivation (microCALl) of calcineurin (CN) in chick DRG neuronal growth cones caused loealizcd filopodial and lamelhpalial collapse and lateral movement away from the irtadiitcd area The relevant downstream substrates of CN for growth cone motility are not known, but Tau, GAP 43 and dynamm are good candidates bas& on: I) their expression in the growth cone, 2) their putative interactions, and 3) calcineurin can regulate these interactions. We reasoned that if a candidate protein were relevant to the action of CN in the growth cone, Ihe growth cone should show a similar behavior in response to the loss of that protein by microCALI. Micro-CAL1 of Tau resulted in similar effect to that observed after micro-CAL1 of CN. Micro-CAL1 of GAP 43 caused lamellipodial retraction as seen during micro-CAL1 of CN but filopodial motility and turning were not affected. MicroCALI of dynamin or using nonimmune antibody had no effect on growth cone behavior. These findings suggest a pathway from localized calcium changes to calcineurin to tau that regulates the cytoskeleton during growth cone motility and axon guidance.