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104 Guidance of retinal axons by repulsive guiding molecules
Author(s) -
Drcscher U.,
Müller H.,
Jay D.,
Bonhoeffer P.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80295-4
Subject(s) - citation , library science , cognitive science , neuroscience , computer science , psychology
Cell density increases Bcl-2, Bcl-x expression and survival of cultured cerebellar granule neurones. Dan Lindholm$g, Susanne HamnCrg , Ute Zirrgiebelz, and Yoko Ohga$(p § Department of Developmental Neuroscience. Biomedxal Center, Box 587, S-75123 Uppsala, Sweden SDepartment of Neurochemistry. Max Pianck Institute for Psychiatry,Am Klopferspitz 18a, D-82152 PlaneggMartinsried, Germany The proto-oncogene bcl-2 and its family members, Bcl-x, and Bax, are recognised as major regulalors of cell death and survival. Although Bcl-2 and Bcl-x are expressed in brain, little is known how they are regulated in neurons. Here we have studied the expression of bcl.2, hcl-xL. and box mRNA in rat cerebellar granule neurons cultured under conditions which influence neuron surwval. Insulin like growth factor-l and brain-derived neurotrophic factor support the survival of these neurons, but affected neilher the expression of bcl-2, bcl-xL nor bax mRNA. In contrast. bcl-2 and bcf-x mRNAs were up-regulated in cerebellar granule neurons plated at high density exhibiting an increased neuronal survival. Western blots showed that cell density also increased Bcl-2 protein levels. However, conditioned medium from dense cultures chd not affect the level of bcl-2 mRNA. This suggests that cell density promotes survival and regulates Bcl-2 expresston in cerebellar granule neurons through an yet unknown signaling pathway.