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61 Glial cell interactions following a traumatic lesion in the CNS: Analysis from control and transgenic rodents
Author(s) -
Privat Alain,
MorinRichaud Claudie
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80256-5
Subject(s) - morin , lesion , neuroscience , genetically modified mouse , transgene , medicine , biology , pathology , genetics , gene
After a traumatic lesion of the spinal cord, regeneration and remyelination of descending tracts may occur only above the lesion, thus permitting a comparision of glial reactivity in hvo different conditions. For that purpose, female Sprague-Dawley rats underwent a complete section of the cord at T7 level, and were sacrificed after 30 min. 1 day, 3 days and 7 days after injury. The cord above and below the lesion was processed for northern blot, in situ hydridization and immunocytochemistry, for the delection of mRNA and corresponding proteins of GFAP, CNPsse and MBP. We found that gliosis is triggered as early as 30 min after lesion, both above and below the section. Conversely, the comparison of CNpase and MBP profiles with counts of immature and mature oligodendrocytes suggests that a sequence of remyelination proceeds uninterrupted above the lesion, whereas it is interrupted below the lesion. Presence of gap junction between oligodendrocytes and astrocytes in the latter location supports unusual glial interaction. Vimentin knock-out mice, in which the astrocyte cytoskeleton is markedly disturbed, underwent the sqe lesion and were sacrificed after 4 days. Preliminary results indicate that the disturbed astrocyte phenotype does not modify astrocyte and oligodendrocyte reactions. In conclusion, spinal cord transection is a suitable model to study neuroglial interactions in relation to regeneration and remyelination in the mammalian CNS. The use of transgenic vim-mice may shed some light on the role of astrocytes in these interactions. Supported by IRME and ARSEP.