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58 Genes involved in neuronal specification at boundary zones in the embyronic zebrafish forebrain
Author(s) -
Wilson Stephen,
Barth K. Anukampa,
Masai Ichiro,
Macdonald Rachel
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80253-x
Subject(s) - citation , forebrain , zebrafish , information retrieval , boundary (topology) , library science , cognitive science , computer science , psychology , biology , neuroscience , gene , genetics , mathematical analysis , mathematics , central nervous system
Development of the chick midbrain, isthmus and cerebellum involves morphogenetic signals produced at the mes-metencephalic boundary. FGFB is a candidate for a secreted protein involved in signaling from this boundary. To test the effect of FGFB on brain development we implanted beads soaked in recombinant FGFS protein into the embryonic chick brain caudal to the zona limitans (ZL). We found that FGFS beads induce ectopic midbrain, isthmic. and cerebellar development and that the type of tissue induced depends on the proximity of the inducer to the ZL. Cerebellum was not induced when the beads were. placed near the ZL (in p2) but was induced when the beads were placed in more caudal tissue, suggesting that the ZL may have signaling properties that suppress ectopic cerebellum induction. In addition we found that whereas beads placed in p2 affect both p2 and the more. caudal prosomere, pl. beads placed m pl oc the mesencephaloo did not affect more rostra1 prosomeres. These observations suggest that the pl/p2 and mes/pl boundaries may act as barriers that prevent rhe rostra1 spread of FGFS induced effects. In summary OUT results suggest thal FGFB plays a central role in the signaling activity of the mes/metencephalic boundary, that prosomere boundaries block the rostra1 spread of FGFB induced effects, and that the ZL inhibits FGFS mediated ectopic cerebellum induction.