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49 Signal pathways involved in neuronal survival promotion by neurotrophic factors
Author(s) -
Courtney M.J.,
Åkerman K.E.O.,
Coffey E.T.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(96)80244-9
Subject(s) - promotion (chess) , neuroscience , neurotrophic factors , neurotrophin , brain derived neurotrophic factor , signal pathway , signal (programming language) , microbiology and biotechnology , biology , signal transduction , political science , genetics , computer science , receptor , politics , law , programming language
Neurotrophic factors (NGF, BDNF, NT-3, NT-415) are members of the neurotrophin family of proteins which support the survival and Induce differentiation of venebrate neurons We have studied the effects of neurotrophic factors on growth cones of developing sensory neurons in order to clarify the mechanisms of specific axonal pathfinding during embryogenesis. NGF and NT-3 appeared to be chemoattractants for growth cones of embryonic dorsal root ganglia (DRG) neurons. BDNF and NT-4/S caused growth cone collapse on NGFor NT-3-dependent DRG neurons but not BDNFor NT4/S-dependent neurons. Quantitative analysis showed that BDNF caused transient decrease of growth rates of NGF-dependenr neurites. BDNF and NT-4 induced 50% decrease of total protein and area of growth cones of NGFor NT-3-dependent neurons, and reorganization of microfilaments: F-actin concentration at the distal part of growth cones decreased 50% after treatment of the growth cones with BDNF for 30 minutes Our results suggest that different neurotrophic factors have chemoattractlve or inhibltory effects on the same growth cone, and they may act as specific growth cone guidance cues: NGF and NT-3 are chemoattractive molecules whereas BDNF and NT415 are having inhibitory effect on growth cone guidance of certain developing neurons. SIGNAL PATHWAYS INVOLVED IN NEURONAL SURVIVAL PROMOTION BY NELJROTROPHIC FACTORS.