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Rapid neurite formation in a human cortical neuronal cell line
Author(s) -
Dunn K.J.,
PerezPolo J.R.,
Wood T.G.
Publication year - 1996
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(95)00073-9
Subject(s) - neurofilament , neurite , cell culture , glial fibrillary acidic protein , immunochemistry , microbiology and biotechnology , enolase , biology , glutamate receptor , cellular differentiation , megalencephaly , chemistry , receptor , neuroscience , biochemistry , immunohistochemistry , in vitro , immunology , antibody , genetics , gene
The subclone HCN‐1 was derived from parental cell lines from cortical tissue of a patient with unilateral megalencephaly growth and immunochemistry staining characteristics [G. V. Ronnett et al. (1990) Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly. Science 248 , 603–605]. As we and others have shown, HCN‐1A cells can be induced to differentiate to a neuronal‐like morphology. HCN‐1A cells stain positively for neurofilament, neuron‐specific enolase and the low‐affinity neurotrophin receptors, p75 NGFR , but not for myelin basic protein, S‐100, or glial fibrillary acidic protein (GFAP). HCN‐1A cells also stain positively for gamma‐aminobutyric acid and glutamate. In the present study, we examine the effects of cell density on the requirements for efficient induction of differentiation of HCN‐1A cells and analyze the time course of this induction and its reversion. We also characterize the changes in cytoskeletal proteins of HCN‐1A cells in response to their differentiation neuronal phenotype.