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Neuronal phenotypes in mouse dorsal root ganglion cell cultures: Enrichment of substance P and calbindin D‐28k expressing neurons in a defined medium
Author(s) -
Ninomiya T.,
BarakatWalter I.,
Droz B.
Publication year - 1994
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/0736-5748(94)90002-7
Subject(s) - dorsal root ganglion , calbindin , substance p , biology , phenotype , chemically defined medium , population , cell culture , microbiology and biotechnology , neuron , in vitro , medicine , immunohistochemistry , sensory system , neuroscience , immunology , biochemistry , neuropeptide , genetics , receptor , gene , environmental health
Abstract The primary sensory neurons in mouse dorsal root ganglia consist of diversified subpopulations which express distinct phenotypic characteristics such as substance P or calbindin D‐28k. To determine whether neuronal phenotypes are altered or not in in vitro cultures carried out in a defined synthetic medium, dissociated dorsal root ganglion cells from newborn mice were grown in the alpha‐modified minimum essential medium either supplemented with 10% fetal calf serum or serum‐free. About 80% of the neurons survived after 5 days of culture in both media, but only 35% or 65% were rescued after 12 days in serum‐free or fetal calf serum supplemented medium, respectively. The neuronal subpopulations expressing substance P or calbindin D‐28k displayed similar morphological properties in both media and a higher resistance to culture conditions than the whole neuronal cell population, especially in serum‐free medium. It is therefore concluded that a defined synthetic medium offers reproducible conditions to culture dorsal root ganglion cells for at least 5 days, stimulates the expression of substance P and enriches preferentially neuronal phenotypes expressing substance P or calbindin D‐28k, for a longer period of culture.